Selasa, 10 Juli 2012 pukul 11:13 am | Ditulis dalam Jurnal, Jurnal Penelitian dan Pengembangan Sains & Humaniora | 6 Komentar
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ISSN 1979-7109
Jurnal Penelitian dan Pengembangan Sains & Humaniora
Volume 4, Nomor 3, Desember 2010


I Nyoman Sukarta & I Dewa Ketut Sastrawidana
Jurusan Pendidikan Kimia FMIPA Undiksha


Penelitian ini bertujuan untuk mengkaji potensi jamur lapuk putih Ganoderma applanatum Isolat Lokal Singaraja untuk merombak zat warna tekstil golongan azo. Jamur Ganoderma applanatum diperoleh dari hutan di Desa Gitgit Kecamatan Sukasada, Kabupaten Buleleng. Tahap pengumpulan data dimulai dari peremajaan pada media potato dextrosa agar (PDA) dan media Czapex cair, uji aktivitas perombakan zat warna tekstil pada media agar, dan uji perombakan pada variasi kondisi pH, konsentrasi zat warna, lama waktu inkubasi dan konsentrasi penambahan nitrogen. Zat warna tekstil yang dirombak adalah remazol red RB dan remazol black B yang sudah umum digunakan sebagai pewarna tekstil. Hasil uji pada media agar menunjukkan jamur lapuk putih Ganoderma applanatum mempunyai kemampuan tinggi untuk merombak zat warna tekstil. Uji aktivitas perombakan pada media cair menunjukkan jamur Ganoderma applanatum mampu merombak kedua zat warna secara maksimum pada konsentrasi 100 mg/L. Perombakan 100 mg/L zat warna remazol red RB dan remazol black B selama 7 hari berlangsung optimum pada pH 5 dengan penambahan 0,5 gram/L NaNO3 sebagai sumber nitrogen dengan efisiensi perombakan secara berturut-turut sebesar 98,46% dan 98,79%.

Kata-kata kunci: ganoderma applanatum, perombakan, remazol red RB, remazol black B


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